| Original Articles : Inoculation route-dependent effects of BCG on asthmatic airway histology |
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Xiang Hua Lin, Inseon S Choi, Young Ah Koh |
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한림의대 한강성심병원 내과 |
| 원저 : 천식성 기도조직에 대한 BCG 투여경로 의존성 효과 |
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림향화, Inseon S Choi, Young Ah Koh |
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| Abstract |
Background : Intranasal (i.n.), as compared with subcutaneous (s.c.), BCG vaccination causes a greater suppression of airway eosinophilia. A comprehensive examination is needed to confirm that in various asthma models.
Methods
: BALB/c mice were immunized with i.n. or s.c. inoculation of BCG 1x105 CFUs. Sensitization and provocaton using ovalbumin (OVA) or Dermatophagoides farinae (Der f) were started at the same time or 1 week after the immunization. And then, a bronchoalveolar lavage (BAL) and an examination of lung tissue using a computerized image analyzer program were performed.
Results
: Both the i.n.- and s.c.- BCG infections reduced eosinophilia in both the BAL fluids and the lung tissues of both OVA- and Der f- asthma models. The proportions of BAL fluid lymphocyte in the mice infected with i.n. BCG were significantly lower than those with s.c. BCG (1.60±0.39% vs. 3.42±0.37%, p<0.01). However, the s.c.- as compared with i.n.- route caused a greater suppression in peribronchial eosinophilia [small (<500 μm) airways: 43.9±12.5 vs 89.5±20.0/mm2, p=0.08; large (>1,000 μm) airways: 17.4±3.2 vs 37.0±5.9/mm2, p<0.05]. The goblet cell proportions in epithelium were also significantly lower in the mice received s.c.- as compared with i.n.- BCG (0.29±0.18 vs 0.43±0.20, p<0.01).
Conclusions
: These results suggest that both i.n.- and s.c.- BCG inoculations reduce eosinophilia in airways, but the s.c. route is more effective in the suppression of the asthmatic responses in lung tissue.(Korean J Med 70:418-428, 2006)
Key Words : Asthma, BCG, Goblet cell, Eosinophils |
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