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Review
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Korean J Med. 2006;71(1):196-196.
- Anti-arthritic effect of OCT-7401, a ginseng extract, on collagen induced arthritis in mice
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Suho Kim, Youngnim Choi, Sunwha Chang, Hyun Ah Kim
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- Anti-arthritic effect of OCT-7401, a ginseng extract, on collagen induced arthritis in mice
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광주기독병원 내과학교실
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- Abstract
- Protein-based anti-TNF-α therapeutics represents a significant advance in the treatment of rheumatoid arthritis(RA), but
drawbacks such as the requirement for repeated administration by injection, antibody formation and the high cost of treatment
pose significant barriers, and the development of orally bioavailable, inexpensive inhibitors of TNF-α represents a highly
desirable strategy. The objective of this study was to show the efficacy of OCT 7401, a ginseng extract, on inhibition of the
in vitro production of TNF-α from peripheral blood monocytes, RA synovial fibroblasts and chondrocytes and on inhibition of
collagen induced arthritis(CIA) in mice. Peripheral blood monocytes were isolated and stimulated with human IFN-γ, LPS or
human recombinant CD40 ligand in the presence of various concentrations of OCT-7401. Cartilage and synovial samples were
obtained from the RA patients, cultured and stimulated with LPS or IL-1 in the presence of various concentrations of OCT-7401.
The concentrations of TNF-α in the culture supernatants were determined by ELISA. CIA was induced in DBA/1J mice. For
prophylactic therapy, OCT7401 was administered from day 20 until day 34 after immunization. For therapeutic studies, animals
with a minimum inflammation score of 1 on day 22 were randomized into treatment groups and OCT 7401 was administered
from day 22 to day 29 after immunization. Histopathologic changes in the whole ankle joints were scored, and the expression
of TNF-α was evaluated by immunohistochemistry. OCT-7401 showed prominent inhibition of TNF-α?upregulation in
peripheral blood monocytes, synoviocytes and chondrocytes induced by various inflammatory stimuli. Prophylactic administration
of OCT7401 resulted in significant amelioration of the clinical arthritis score in CIA mice. OCT-7401 not only decreased the
clinical arthritis score, but also delayed the onset of arthritis significantly. OCT-7401 reduced cell infiltration into synovial tissues
and reduced TNF-α expression in infiltrating inflammatory cells. Our results suggest that OCT-7401 is one viable and feasible
approach to the treatment of RA or other diseases characterized by upregulation of TNF-α upregulation.
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